Research:2026-06-18/Journal article/seed-compositional-profiling-of-21-industrial-hemp-varieties-and-comparative-proteomic-analysis-of-wild-and-cu

Research record18 June 2026Journal article

Seed Compositional Profiling of 21 Industrial Hemp Varieties and Comparative Proteomic Analysis of Wild and Cultivated Cannabis sativa L. Genotypes

Kusum Raj Tamang, Brian P. Mooney, Thomas P. Mawhinney, Joshua Asiamah, Swastika Sharma, Shreesha Padyana, Christian Carson, Prabesh Koirala, Sakina Mahdi, Babu Valliyodan

Journal of Proteome Research · 2026

Abstract

Industrial hemp is a nutritious crop with wide implications. We conducted a comprehensive study of amino acid, fatty acid, and sugar profiles, which have a profound impact on human nutrition and animal feed. We found that variety significantly influences the nutritional profile of hemp. Furthermore, we found glutamic acid, arginine, and aspartic acid to be the primary amino acids; linoleic acid was the major fatty acid, and fructose and sucrose were the dominant sugars. Proteomic analysis identified 240 differential proteins (DEPs) in wild and cultivated dual-type varieties and 648 in wild and fiber-type hemp varieties. We found that all the varieties shared common proteins, indicating a strong seed proteomic profile in the domesticated and wild-type varieties. Notably, most of the differentially expressed proteins were downregulated in cultivated types compared to their wild counterparts. Overall, this study elucidates the nutritional and proteomic profiles of diverse hemp varieties, providing fundamental insights into the molecular and genetic changes associated with the domestication and cultivation of hemp.

The paper's abstract, reproduced as published.

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Record created 8 August 2026 · maintained by landrace.wiki editors · page history

Published18 Jun 2026

TypeJournal article

VenueJournal of Proteome Research

Year2026

DOI10.1021/acs.jproteome.5c00554

DOIPDF

2026-06-18 2026-08-08 Seed Compositional Profiling of 21 Industrial Hemp Varieties and Comparative Proteomic Analysis of Wild and Cultivated Cannabis sativa L. Genotypes Journal article Chemistry Kusum Raj Tamang, Brian P. Mooney, Thomas P. Mawhinney, Joshua Asiamah, Swastika Sharma, Shreesha Padyana, Christian Carson, Prabesh Koirala, Sakina Mahdi, Babu Valliyodan Journal of Proteome Research 2026 10.1021/acs.jproteome.5c00554 https://doi.org/10.1021/acs.jproteome.5c00554 https://pmc.ncbi.nlm.nih.gov/articles/PMC13340426/pdf/pr5c00554.pdf Profiles seed composition across 21 industrial hemp varieties and compares the seed proteomes of wild, cultivated dual-purpose and fibre-type Cannabis sativa genotypes. Between 240 and 648 proteins were differentially expressed depending on the comparison, most of them downregulated in cultivated material relative to wild. The result gives molecular evidence of proteomic divergence associated with domestication. Industrial hemp is a nutritious crop with wide implications. We conducted a comprehensive study of amino acid, fatty acid, and sugar profiles, which have a profound impact on human nutrition and animal feed. We found that variety significantly influences the nutritional profile of hemp. Furthermore, we found glutamic acid, arginine, and aspartic acid to be the primary amino acids; linoleic acid was the major fatty acid, and fructose and sucrose were the dominant sugars. Proteomic analysis identified 240 differential proteins (DEPs) in wild and cultivated dual-type varieties and 648 in wild and fiber-type hemp varieties. We found that all the varieties shared common proteins, indicating a strong seed proteomic profile in the domesticated and wild-type varieties. Notably, most of the differentially expressed proteins were downregulated in cultivated types compared to their wild counterparts. Overall, this study elucidates the nutritional and proteomic profiles of diverse hemp varieties, providing fundamental insights into the molecular and genetic changes associated with the domestication and cultivation of hemp.