Research:2026-03-30/Journal article/evaluation-of-downy-mildew-resistance-in-the-usda-hemp-germplasm-repository-using-high-throughput-and-traditio
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Evaluation of Downy Mildew Resistance in the USDA Hemp Germplasm Repository Using High-Throughput and Traditional Phenotyping Methods
Plant Disease
Screens 108 hemp entries from the USDA Hemp Germplasm Repository for resistance to downy mildew, caused by Pseudoperonospora cannabina and first identified in New York in 2020. Three methods were compared: robotic imaging with convolutional neural network analysis of leaf discs, visual rating of the same discs and detached-leaf assays. Severity ranged from 0 to 100%, and two entries, G 33532 and the cultivar Santhica 27, showed none under all three methods. The authors find the imaging approach promising but in need of refinement, since it underestimated severity against visual scoring. The paper names resistant material held in a public germplasm collection.