Research:2026-02-09/Journal article/genome-wide-inventory-and-functional-characterization-of-cannabinoid-oxidocyclases-in-cannabis-sativa

ResearchJournal article9 February 2026

Genome-wide inventory and functional characterization of cannabinoid oxidocyclases in Cannabis sativa

Minxuan Li, Sen Cai, Xu Bai, Yuzeng Ouyang, Lina Chen, Zhiyuan Zhang, Weiqi Tang, Marc Xu, Deyong Ao, Yaxing Li, Yunwei Sun, Liette Vasseur, Shijun You, Yang Liu, Nirit Bernstein, Yong Wang, Yuanyuan Liu

Horticulture Research

Surveys five cannabis genomes for cannabinoid oxidocyclases, the enzymes that convert CBGA into THCA, CBDA and CBCA, and identifies 36 putatively functional genes in five clades, including a divergent gene the authors name THCAS-like 1 (TASL1). Expression in mature glandular trichomes was profiled across nine varieties and correlated with cannabinoid content across 21. Assays in Pichia pastoris and Nicotiana benthamiana showed TASL1 and CBDAS-like 1 producing mainly THCA and CBDAS-like 2 varying with the host, and substitutions in an oxygen-reactivity motif raised the catalytic efficiency of CBDAS up to threefold. The paper is a short research letter.

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DOI10.1093/hr/uhag023

Record created 15 October 2026maintained by landrace.wiki editorspage history

2026-02-09 2026-10-15 Genome-wide inventory and functional characterization of cannabinoid oxidocyclases in Cannabis sativa Journal article Genetics Minxuan Li, Sen Cai, Xu Bai, Yuzeng Ouyang, Lina Chen, Zhiyuan Zhang, Weiqi Tang, Marc Xu, Deyong Ao, Yaxing Li, Yunwei Sun, Liette Vasseur, Shijun You, Yang Liu, Nirit Bernstein, Yong Wang, Yuanyuan Liu Horticulture Research 2026 10.1093/hr/uhag023 https://doi.org/10.1093/hr/uhag023 Surveys five cannabis genomes for cannabinoid oxidocyclases, the enzymes that convert CBGA into THCA, CBDA and CBCA, and identifies 36 putatively functional genes in five clades, including a divergent gene the authors name THCAS-like 1 (TASL1). Expression in mature glandular trichomes was profiled across nine varieties and correlated with cannabinoid content across 21. Assays in Pichia pastoris and Nicotiana benthamiana showed TASL1 and CBDAS-like 1 producing mainly THCA and CBDAS-like 2 varying with the host, and substitutions in an oxygen-reactivity motif raised the catalytic efficiency of CBDAS up to threefold. The paper is a short research letter.